anti b7 h3 antibody (R&D Systems)
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Structured Review
R&D Systems
anti b7 h3 antibody
Anti B7 H3 Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 101 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/b7+h3/Human+B7-H3+Antibody/bio_rxiv__64898__2026__04__15__718753-197-2-5
Average 95 stars, based on 101 article reviews
Anti B7 H3 Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 101 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/b7+h3/Human+B7-H3+Antibody/bio_rxiv__64898__2026__04__15__718753-197-2-5
Average 95 stars, based on 101 article reviews
anti b7 h3 antibody - by Bioz Stars,
2026-10
95/100 stars
Images
1) Product Images from "Transcription Factor–Mediated Reprogramming of Cancer-Associated Fibroblasts Reveals Targetable Vulnerabilities in Solid Tumors"
Article Title: Transcription Factor–Mediated Reprogramming of Cancer-Associated Fibroblasts Reveals Targetable Vulnerabilities in Solid Tumors
Journal: bioRxiv
doi: 10.64898/2026.04.15.718753
Figure Legend Snippet: A. Immunocytochemistry (ICC) analysis demonstrates strong B7-H3 (CD276) expression on both PC-3 cancer cells and pCAFs, visualized by red fluorescence. B. In 2D co-culture assays, anti-B7-H3 CAR T cells exhibit antigen-specific cytotoxicity against PC-3 cells, with killing efficiency increasing across graded effector-to-target (E:T) ratios.
Techniques Used: Immunocytochemistry, Expressing, Fluorescence, Co-Culture Assay
Figure Legend Snippet: Stromal Effects on CAR T-Cell Activity in 3D-Bioprinted PC-3 Spheroids. A. Representative images of stromal populations influencing anti-B7-H3 CAR T-cell killing function . B. PC-3 spheroid with pCAF/rpNFs interaction with CAR-T cells monitored by IncuCyte imaging system. C. The killing effects of B7-H3 CAR T cells on PC-3 cells in day 5 compared to day 1. D . Imaging of live cells at different time.
Techniques Used: Activity Assay, Imaging
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Immunohistochemistry:Article Title: A clinicopathological analysis of supratentorial ependymoma, ZFTA fusion-positive: utility of immunohistochemical detection of CDKN2A alterations and characteristics of the immune microenvironment. Article Snippet: .. IHC was performed on 3–4-μm-thick sections from formalin-fixed, paraffin-embedded (FFPE) tissue blocks as follows: the primary antibodies used included those for CD3 (PS1, 1: 100, Novocastra, Newcastle Upon Tyne, UK), CD4 (1F6, 1: 40, Novocastra, Newcastle Upon Tyne, UK), CD8 (C8/144B, 1: 50, Dako, Santa Clara, CA, USA), CD20 (L26, 1: 100, Dako, Santa Clara, CA, USA), MTAP (2G4, 1: 200, 1 3 Abnova, Taipei, Taiwan), p16 (G175-405, 1: 10, BD Biosciences, Franklin Lakes, NJ, USA), CD204 (SRA-E5, 1: 25, Trans Genic Inc., Fukuoka, Japan), Iba-1 (013-27691, 1: 1000, Fujifilm Wako, Osaka, Japan), NFκB p65 (D14E12, 1:400, Cell Signaling Technology, Inc., Danvers, MA, USA), and Article Title: Correlation of expression of Major Vault Protein with androgen receptor and immune checkpoint protein B7-H3, and with poor prognosis in prostate cancer. Article Snippet: .. Immunohistochemistry (IHC) was carried out using the following primary antibodies: MVP (Abcam [1032], ab14562), dilution: 1:60; PTEN (Merck Millipore, clone 6H2.1), dilution 1:100; Article Title: A clinicopathological analysis of supratentorial ependymoma, ZFTA fusion-positive: utility of immunohistochemical detection of CDKN2A alterations and characteristics of the immune microenvironment Article Snippet: .. IHC was performed on 3–4-μm-thick sections from formalin-fixed, paraffin-embedded (FFPE) tissue blocks as follows: the primary antibodies used included those for CD3 (PS1, 1: 100, Novocastra, Newcastle Upon Tyne, UK), CD4 (1F6, 1: 40, Novocastra, Newcastle Upon Tyne, UK), CD8 (C8/144B, 1: 50, Dako, Santa Clara, CA, USA), CD20 (L26, 1: 100, Dako, Santa Clara, CA, USA), MTAP (2G4, 1: 200, Abnova, Taipei, Taiwan), p16 (G175-405, 1: 10, BD Biosciences, Franklin Lakes, NJ, USA), CD204 (SRA-E5, 1: 25, Trans Genic Inc., Fukuoka, Japan), Iba-1 (013-27691, 1: 1000, Fujifilm Wako, Osaka, Japan), NFκB p65 (D14E12, 1:400, Cell Signaling Technology, Inc., Danvers, MA, USA), and Formalin-fixed Paraffin-Embedded:Article Title: A clinicopathological analysis of supratentorial ependymoma, ZFTA fusion-positive: utility of immunohistochemical detection of CDKN2A alterations and characteristics of the immune microenvironment. Article Snippet: .. IHC was performed on 3–4-μm-thick sections from formalin-fixed, paraffin-embedded (FFPE) tissue blocks as follows: the primary antibodies used included those for CD3 (PS1, 1: 100, Novocastra, Newcastle Upon Tyne, UK), CD4 (1F6, 1: 40, Novocastra, Newcastle Upon Tyne, UK), CD8 (C8/144B, 1: 50, Dako, Santa Clara, CA, USA), CD20 (L26, 1: 100, Dako, Santa Clara, CA, USA), MTAP (2G4, 1: 200, 1 3 Abnova, Taipei, Taiwan), p16 (G175-405, 1: 10, BD Biosciences, Franklin Lakes, NJ, USA), CD204 (SRA-E5, 1: 25, Trans Genic Inc., Fukuoka, Japan), Iba-1 (013-27691, 1: 1000, Fujifilm Wako, Osaka, Japan), NFκB p65 (D14E12, 1:400, Cell Signaling Technology, Inc., Danvers, MA, USA), and Article Title: A clinicopathological analysis of supratentorial ependymoma, ZFTA fusion-positive: utility of immunohistochemical detection of CDKN2A alterations and characteristics of the immune microenvironment Article Snippet: .. 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Antibodies for western blot analysis are as follows: Article Title: Impact of B7-H3 expression on metastasis, immune exhaustion and JAK/STAT and PI3K/AKT pathways in clear cell renal cell carcinoma Article Snippet: Total cell lysates were obtained lysing cells in the M-PER mammalian protein extraction reagent (Thermo Fisher Scientific) supplemented with phosphatase and protease inhibitor cocktails (PhosSTOP and cOmplete; Roche). .. Immunoblot analysis of Article Title: Impact of B7-H3 expression on metastasis, immune exhaustion and JAK/STAT and PI3K/AKT pathways in clear cell renal cell carcinoma Article Snippet: Total cell lysates were obtained lysing cells in the M-PER mammalian protein extraction reagent (Thermo Fisher Scientific) supplemented with phosphatase and protease inhibitor cocktails (PhosSTOP and cOmplete; Roche). .. Immunoblot analysis of SDS Page:Article Title: Impact of B7-H3 expression on metastasis, immune exhaustion and JAK/STAT and PI3K/AKT pathways in clear cell renal cell carcinoma Article Snippet: Total cell lysates were obtained lysing cells in the M-PER mammalian protein extraction reagent (Thermo Fisher Scientific) supplemented with phosphatase and protease inhibitor cocktails (PhosSTOP and cOmplete; Roche). .. Immunoblot analysis of Article Title: Impact of B7-H3 expression on metastasis, immune exhaustion and JAK/STAT and PI3K/AKT pathways in clear cell renal cell carcinoma Article Snippet: Total cell lysates were obtained lysing cells in the M-PER mammalian protein extraction reagent (Thermo Fisher Scientific) supplemented with phosphatase and protease inhibitor cocktails (PhosSTOP and cOmplete; Roche). .. Immunoblot analysis of Staining:Article Title: Chimeric antigen receptor polypeptides and methods of using same Article Snippet: Materials Asunaprevir (ASV), danoprevir (DPV), simeprevir (SPV), and grazoprevir (GPV) were purchased from ApexBio. .. Cell surface markers were stained with mouse monoclonal antibodies targeting PD-1 (PE-Cy7, eBioscience), LAG3 (PE, eBioscience), Tim-3 (BV 510, BioLegend), and Bioprocessing:Article Title: Chimeric antigen receptor polypeptides and methods of using same Article Snippet: Materials Asunaprevir (ASV), danoprevir (DPV), simeprevir (SPV), and grazoprevir (GPV) were purchased from ApexBio. .. Cell surface markers were stained with mouse monoclonal antibodies targeting PD-1 (PE-Cy7, eBioscience), LAG3 (PE, eBioscience), Tim-3 (BV 510, BioLegend), and |
